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Fig. 5 | Chinese Medicine

Fig. 5

From: Cyp4a12-mediated retinol metabolism in stellate cells is the antihepatic fibrosis mechanism of the Chinese medicine Fuzheng Huayu recipe

Fig. 5

The retinol metabolism in HSCs was regulated by FZHY treatment in vitro. Primary hepatic stellate cells were isolated and cultured in vitro through normal C57BL/6 mice, which were spontaneously activated on Day 1, Day 4, and Day 7. A Oil red staining and immunofluorescence staining of α-SMA (red immunofluorescence) in HSCs. B Dynamic observation of the relative mRNA expression levels of retinoic acid metabolism-related genes in HSCs. C Determination of a nontoxic concentration of FZHY. D–E Pharmacodynamic observation of the relative mRNA expression levels of retinoic acid metabolism-related genes in HSC cultured for 4 days and 7 days. F The immunofluorescence staining of Desmin (red immunofluorescence) and Rae-1 (green immunofluorescence). Values represent the means ± SDs

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