Fig. 5From: Cyp4a12-mediated retinol metabolism in stellate cells is the antihepatic fibrosis mechanism of the Chinese medicine Fuzheng Huayu recipe The retinol metabolism in HSCs was regulated by FZHY treatment in vitro. Primary hepatic stellate cells were isolated and cultured in vitro through normal C57BL/6 mice, which were spontaneously activated on Day 1, Day 4, and Day 7. A Oil red staining and immunofluorescence staining of α-SMA (red immunofluorescence) in HSCs. B Dynamic observation of the relative mRNA expression levels of retinoic acid metabolism-related genes in HSCs. C Determination of a nontoxic concentration of FZHY. D–E Pharmacodynamic observation of the relative mRNA expression levels of retinoic acid metabolism-related genes in HSC cultured for 4 days and 7 days. F The immunofluorescence staining of Desmin (red immunofluorescence) and Rae-1 (green immunofluorescence). Values represent the means ± SDsBack to article page