Fig. 6From: Cyp4a12-mediated retinol metabolism in stellate cells is the antihepatic fibrosis mechanism of the Chinese medicine Fuzheng Huayu recipe The Rae-1 expression was restored under the FZHY treatment by regulating Cyp4a12a/b in vitro. HSCs were isolated from healthy control mice and adapted in culture for 1 day, and then, adenovirus was used to transfect 1 day HSCs for 48 h, as described in Materials and Methods. A The Cyp4a12a/b inhibition rates were observated through quantitative real-time PCR assays. B Pharmacodynamic observation of Cyp4a12a/b mRNA expression levels in adenovirus-transfected HSCs. C Pharmacodynamic observation of Rae-1α/γ/δ/ε mRNA expression levels in adenovirus-transfected HSCs. D, E Pharmacodynamic observation of relative mRNA expression levels of retinoic acid metabolism-related genes and nuclear receptors in adenovirus-transfected HSCs. Values represent the means ± SDsBack to article page