Fig. 1

Experimental protocol of the study. Rats were randomly divided into 5 groups after one week of adaptive feeding. Expect for the control group, all animals received subcutaneous injection of D-gal (150 mg/kg) for 10 weeks. The rats in the BSYZ-L, BSYZ-H and DNP groups received oral gavage every day in the third week, and last into the tenth week. The behavioral tests, including morris water maze, open field test and novel object recognition task) were performed during the ninth and tenth weeks. After the behavioral tests, the animals were sacrificed and brain tissue was collected for experimental analysis