Fig. 3
From: Tanshinone IIA inhibits osteoclastogenesis in rheumatoid arthritis via LDHC-regulated ROS generation
The effects of Tan IIA on RANKL-induced osteoclast differentiation and bone resorption in vitro. A Anti-tartrate phosphatase (TRAP)-stained sections of RANKL-induced osteoclasts. B Detection of RANKL-induced actin ring formation in osteoclasts. Immunofluorescence staining was used to identify the actin rings (red). Nuclei were stained with DAPI (blue). C Fusion indices of osteoclasts (multinucleated TRAP-positive cells). D The proportion of actin ring formation (data are mean ± SD, n = 3 for C and D, *P < 0.05, ***P < 0.001 vs. RANKL group)