Dianthi herba: a comprehensive review of its botany, traditional use, phytochemistry, and pharmacology

Dianthi herba (called “Qumai” in Chinese) is the dried aerial part of Dianthus superbus L. and Dianthus chinensis L. The species are mainly distributed in the temperate and warm temperate regions in the northern hemisphere, and some regions in Africa and Oceania, as well as South America. However, the distribution pattern of Dianthi herba has not been reviewed. In this review, we summarize the research progress on the botany, traditional use, phytochemistry, pharmacology, toxicology, and clinical applications of Dianthi herba. Approximately 194 chemical compounds have been identified and isolated from Dianthi herba, the most important being triterpenoid saponins, flavonoids, and volatile oil compounds. These compounds possess antiviral, anticancer, antioxidant, and antimicrobial properties, inter alia. Further studies should be carried out on Dianthi herba to elucidate more of its active principles and their mechanisms of action. Supplementary Information The online version contains supplementary material available at 10.1186/s13020-022-00570-2.


Background
Dianthi herba is a traditional Chinese medicine (TCM) known as "Qumai. " According to the Pharmacopoeia of the People's Republic of China (PRC; 2020), the authentic varieties of Dianthi herba are derived from the dried aerial parts of Dianthus superbus L. and Dianthus chinensis L., belonging to the family Caryophyllaceae, known as "Qumai" and "Shizhu" (Pharmacopoeia Commission of PRC, 2020), respectively. The first record of Dianthi herba is in Shennong Bencaojing (the Classic of Herbal Medicine). Traditionally, it has been used to cure diuresis, invigorate blood circulation, and regulate menstruation [1,2]. Owing to its good efficacy, Dianthi herba is shown that Dianthi herba has antitumor, antioxidant, antiviral, anti-inflammatory, diuretic, uterine excitatory, antimicrobial, and neuroprotective properties [5,6].
In this paper, we used PubMed, ScienceDirect, Web of Science, Springer, Wiley, and the China National Knowledge Infrastructure (CNKI) to collect the relevant literature on Dianthi herba from 1959 to 2021 and reviewed its botany, traditional application, phytochemistry, pharmacological properties, toxicity, and clinical applications ( Fig. 1 visually shows the thinking of this paper). We aimed to provide a comprehensive review of Dianthi herba to determine its therapeutic potential and indicate directions for future research that will serve as a basis for the further development and utilization of this resource.

Botany
The family Caryophyllaceae comprises approximately 2000 plant varieties distributed worldwide. D. superbus is mainly distributed in the temperate and warm temperate regions of the northern hemisphere, particularly in northern Europe, central Europe, Siberia, Kazakhstan, Mongolia (western and northern), Korea, Japan, and China, and in some regions in Africa and Oceania, as well as South America (Fig. 2). D. chinensis inhabits regions similar to those of D. superbus and is mainly distributed in Kazakhstan, Korea, Mongolia, Russia, and Europe (Fig. 3). The species distribution data are from the Global Biodiversity Information Facility (GBIF)-is an international network and data infrastructure funded by the world's governments and aimed at providing anyone, anywhere, open access to data about all types of life on Earth. GBIF Secretariat currently manages and maintains GBIF.
Dianthus superbus is an herbaceous perennial plant, growing to a height of 50-60 cm with stems that are clumped and branched at the upper part. The leaf blades are linear-lanceolate, 5-10 × 0.3-0.5 cm in size, and the apex is acuminate and connate at the base forming a sheath. There are one to two terminal or axillary flowers with two to three pairs of bracts that are obovate and 0.6-1 × 0.4-0.5 cm in size. The apex has a long cusp, while the upper edge of the petal is lobed into a fringed shape. The plants blossom from June to September, and the fruit appears from August to October. This plant is widely cultivated in China where it grows in hilly mountain forests, forest margins, meadows, valleys, and other locations at altitudes of 400-3700 m (Flora Reipublicae Popularis Sinicae, 2019).
Dianthus chinensis is an herbaceous perennial plant that grows to a height of 30-50 cm, and the whole plant is hairless. The stems arise from rhizomes and are sparsely clustered with erect upper branches. The leaf blades are linear-lanceolate and 3-5 × 0.2-0.4 cm in size, with an apex that is acuminate and connate at the base forming   57.6% in Europe, and 4.1% in North America and 3.2% in South America. More detailed coordinate information shows this in more detail (see Additional file 1) a sheath. The flowers are solitary branchlets that are terminal or compound umbels and approximately 1-3 cm long. There are four bracts, while the length of the sepal is half, the apex has a long cusp, and the upper edge of the petal is tooth-shaped. The plant blossoms from May to June, and the fruits appear from July to September. This plant is native to northern China and currently grows in northern and southern regions, grasslands, hillside meadows, and other regions (Flora Reipublicae Popularis Sinicae, 2019). The plant morphologies of D. superbus and D. chinensis are shown in Fig. 4.

Traditional uses
TCMs have been used to treat various diseases, and the long history of use and superior safety profile of TCM formulations have increased the confidence of public in TCMs and its acceptance. Dianthi herba is one of the few commonly used TCMs, and is recorded in ancient Chinese books and Chinese herbal medicine manuals in successive dynasties. It has been documented under different names in several well-known medicinal records, including Shennong Bencaojing (called "Jujumai"), Guang Ya (called "Ziwei"), Ri Hua Zi Ben Cao (called "Shizhu". This is the name of "Shizhu" for the first time.), and Qi Min Yao Shu (called "Dimian", because its seeds can be made into cakes to eat), and in contemporary medicinal records, such as Zhong Hua Ben Cao and The Chinese Dictionary of Herbal Medicine. Qumai is a combination of seeds, stems, and leaves and was first mentioned in Shennong Bencaojing. The textual legend of the plant is shown in Ben Cao Jing Ji Zhu, the plant is described to have a fruit resembling wheat. The plant morphology of a carnation "stem with distinct nodes, leaves opposite, striate, bract subcalyx, petal margins shallowly toothed" has been accurately described in the book of Zhi Wu Ming Shi Tu Kao. Since the Liang Dynasty, Dianthus has been used as a medicinal herb in the name of "Qumai, " which has become the main plant source of the TCM "Qumai. " The detailed traditional uses are shown in Table 1.
Ancient books have described the characteristics of Dianthi herba. Dianthi herba tastes bitter, and is cold and non-toxic. Dianthi herba is mainly used as a diuretic to help relieve the pain during urination. Its clinical uses include the treatment of urinary tract infections, red and astringent urination, dysmenorrhea, red eye, eye swelling and pain, esophageal cancer, and rectal cancer. Dianthi herba has a wide range of medicinal uses and is mostly prescribed clinically (Table 2). Furthermore, Dianthi herba is used as medicine by ethnic minorities in China. Dianthi herba is recorded in Zhonghua Materia Medica-Mongolian Medicine Volume; it is indicated for blood fever, blood tingling, liver fever, and other diseases Qumai, " also known as "Jujumai", "Daju" and "Yuemai. " In ancient times, it was mainly distributed in Shandong, Shanxi, Henan, and Huaihe River Basins, with Xinjiang County and its surrounding areas in Shanxi Province as its authentic production areas; D. chinensis depicted in Zhi Wen Ben Cao (B): It was called "Shiyangjin" in ancient times, and was born in the wilderness. It sprouts in spring and blossoms in summer. The bud is very small and has slender flowers; The plant of D. superbus (C), and the plant of D. chinensis (D) such as phase stroke and puerperal fever [4]. In China, Dianthi herba is sold commercially based on the external features of the dried aerial part. Currently, most medicinal products of Dianthi herba sold in the market are sourced from D. chinensis and D. superbus, but there are also products containing adulterants, such as D. chinensis var. versicolor and D. superbus var. longicalycinus [7][8][9].

Flavonoids
Flavonoids are an important class of natural organic compounds with a basic 2-phenyl-chromone structure; they are widely distributed in the plant kingdom. Most natural flavonoids exist in the form of glycosides, which differ in composition according to the type, quantity, linkage position, and connection mode of the sugar. A few flavonoids and flavonoid glycosides have been reported in D. superbus and D. chinensis.

Phenolic acids
A total of 15 phenolic acids were isolated. Including
A study investigated the apoptotic effects of the ethanol extract of D. chinensis (EEDC) in the human hepatocellular carcinoma cells HepG2 and found that treatment with 50, 100, 200, and 400 µg/mL EEDC These results support a mechanism in which D. chinensis induces apoptosis through the mitochondrial pathway and caspase activation in HepG2 cells [56].
In conclusion, extracts of the effective parts of Dianthi herba have antitumor biological activity, which is of importance in explaining the material basis of the antitumor efficacy of Dianthi and provides evidence for the clinical use of Dianthus spp. in the treatment of tumor. However, it is necessary to strengthen research on the active ingredients and anticancer activity of Dianthi in vivo to provide a theoretical basis for the development and application of Dianthi.
In addition, Chen et al. used a Wistar rat model to examine the effect of Qumai decoction on lipid peroxidation in liver homogenates [82]. At a concentration of 3.13 mg/mL, the decoction had obvious inhibitory effects on lipid peroxidation in rat liver homogenates (P < 0.001) [61]. The antioxidant activity of components of different solvent fractions of the extract of D. superbus was studied, and each component exhibited varying inhibitory effects on free radicals. Components of the ethyl acetate (EtOAc) fractions exhibited a more potent effect than those of other fractions, and the IC 50 indicated a strong antioxidant activity at 1.25 mg/mL [97,98]. Furthermore, the scavenging effect of all components of the EtOAc fraction on hydroxyl radicals was significant [62].
The antioxidant activity of Dianthus is exerted through different mechanisms by scavenging free radicals and by upregulating factors related to the Nrf2/ARE signaling pathway; these findings provide a preliminary basis for the development of new natural antioxidants and the further development of anti-aging drugs [63,64]. Therefore, although the antioxidant activity of D. superbus has been widely studied, it is necessary to further study the mechanism through which it reduces reactive oxygen species (ROS) and free radical formation, to provide a detailed theoretical basis for the practical application of D. superbus.

Antiviral activity
Nile et al. studied the antiviral and cytotoxic activities of quercetin 3-glucoside (Q3G) isolated from D. superbus against influenza virus infection [22]. To determine the mechanism underlying the antiviral effect of Q3G on the influenza virus, time-dependent antiviral tests, molecular docking studies, virus-induced symptom analysis, pre-incubation, and screening of related gene expression were conducted [99]. Q3G from D. superbus showed strong antiviral activity against influenza A and B viruses. The IC 50 value against A/PR/8/34, A/Victoria/3/75, A/ WS/33, B/Maryland/1/59, and B/Lee/40 was 4.93, 6.43, 9.94, 8.3, and 7.1 μg/mL, respectively. It inhibited virusinduced cellular ROS generation and acidic vesicular organelles formation. Moreover, Q3G and oseltamivir, administered to the control group, were found to be cytotoxic [22]. The half-maximal cytotoxic concentration (CC 50 ) value was > 100 μg/mL and nontoxic. In addition, Q3G did not inhibit the neuraminidase (NA) activity of the influenza virus but blocked viral replication, showing a more competitive binding affinity (− 8.0 kcal/mol) than guanosine triphosphate (GTP) (− 7.0 kcal/mol) in blocking the Pb2 subunit of influenza viral polymerase. Therefore, Q3G has a positive protective effect on infected host cells, and a strong inhibitory effect on influenza A and B viruses, which provides a new research direction on Q3G in the development of anti-influenza drugs.
Quercetin-7-O-glucoside (Q7G), a compound isolated from D. superbus, was analyzed for inhibitory effects on viral RNA replication using quantitative RT-PCR. The blocking effect of Q7G on the basic protein subunits of the RNA polymerase of influenza virus was detected using the AutoDock Vina program and M7GTP using a computer molecular docking assay. Q7G showed no cytotoxicity in MDCK cells (CC 50 > 100 μg/mL), and its IC 50 value was 3.1, 6.61, 8.19, and 5.17 μg/mL against influenza A/PR/8/34, A/Vic/3/75, B/Lee/40, and B/Maryland/1/59 virus strains, respectively. Treatment with Q7G highly reduced ROS and autophagy formation induced by influenza virus infection. Q7G does not bind directly to virus particles or affect NA activity. These results indicate that Q7G inhibits RNA polymerase and occupies the binding site of M7GTP on Pb2 [27]. In conclusion, D. superbus has strong antiviral activity as a TCM, especially the active compounds Q3G and Q7G. These findings provide a direction for further research on D. superbus as an antiviral drug.

Anti-inflammatory activity
Shin et al. evaluated the anti-inflammatory effects of D. superbus fructus ethanolic extract (DSE) (200 mg/kg) on Th2-type cytokines, eosinophil infiltration, and other factors in an ovalbumin (OVA)-induced, murine asthma model [65]. To elucidate the potential mechanism of the anti-inflammatory effect of DSE, the expression of inducible nitric oxide synthase (iNOS) in the respiratory tract was also analyzed. DSE significantly reduced the levels of interleukin (IL)-4, IL-13, eotaxin, and immunoglobulin E (IgE); the number of inflammatory cells in the bronchoalveolar lavage fluid (BALF); level of inflammatory cell infiltration; and mucus production in the respiratory tract [100]. The results indicate that DSE also attenuates the overexpression of iNOS induced by OVA challenge. This suggests that DSE effectively protects against allergic airway inflammation by downregulating iNOS expression, and it has potential as a therapeutic agent for allergic asthma [65,66].
In another study, to discover novel immunosuppressants, cytokine enzyme-linked immunosorbent spot and enzyme-linked immunosorbent assays were used to verify the dichloromethane soluble component of D. superbus (50 μg/mL) for suppressive effects against human alloreactive T cells [101]. The addition of D. superbus extracts to human mixed lymphocyte cultures caused a dose-dependent inhibition of proliferation and interferon c (IFNc) production by memory alloreactive T cells and increased the proportion of forkhead box P3-positive (Foxp3 + ) CD4 + T cells. To determine whether the extracts of D. superbus induce regulatory T cells, anti CD3/CD28-stimulated CD4 T cells were treated, and a dose-dependent upregulation of Foxp3 was found to be associated with novel suppressive effects. Mechanistically, extracts of D. superbus did not induce T cell IL-10 or transforming growth factor beta (TGFβ), but they blocked T cell protein kinase B (Akt) phosphorylation, a key signaling nexus required for T cell proliferation and expansion that simultaneously prevents Foxp3 transcription. This study provides novel insights into the antiinflammatory effects of D. superbus [67].
Slotkin et al. reported that lipophilic organic acids (20 μg/mL) and intermediate lipophilic organic acids (20 μg/mL) in aqueous extracts of D. superbus were highly effective in reducing IgE secretion by a human B cell line. Furthermore, preliminary in vivo studies showed that D. superbus reduced the symptoms of anaphylactic shock in peanut-sensitized mice [68].
Although basic animal studies are still needed to investigate the anti-inflammatory effect of D. superbus, its anti-inflammatory effect and potential action mechanism provide a basis for its potential application in the development of new drugs. In addition, its anti-inflammatory activity can be enhanced when it is used in combination with other drugs as a combined supplement [69].

Diuretic activity
In selected hospitals, from 2007 to 2014, Xu et al. treated 200 patients with ureteral calculi who were allocated to the control and treatment groups using the random number table method [70], with 100 patients in each group. The patients in the control group were administered extracorporeal shock wave lithotripsy combined with rehydration, spasmolysis, and diuretic therapy. The results suggested that D. superbus decoction combined with extracorporeal shock wave lithotripsy for the treatment of ureteral calculi required a short treatment time and that the patients exhibited a low recurrence rate. This finding suggests that this treatment may be suitable for widespread clinical application [70]. Li et al. gavaged rabbits with Qumai decoction (2 g/kg), and after 60 min, the urinary output increased significantly, demonstrating an improved diuretic effect [71]. Deng et al. prepared a compound decoction containing D. superbus, and the effect was investigated using laboratory examinations such as routine urine analysis as well as clinical symptom evaluation. The results showed that the urinary tract symptoms were relieved following treatment with the decoction [72]. Other studies have proven that prescription drugs containing D. superbus exhibit diuretic effects that improve urinary tract symptoms [71,73,74].

Uterine excitatory activity
Li et al. used a mouse model of early gestation to evaluate the effects of various doses of fruit extracts of D. superbus on embryo number and growth [75]. Mice in the gestation period were intragastrically administered various doses (8.5, 17, and 34 g/kg) of the extract once a day, and fruit extracts of D. superbus showed obvious effects on early pregnancy in mice. The fruit extract of D. superbus at the three doses increased the abortion rate in a dose-dependent manner. The anti-pregnancy effects of the fruit may be mediated by a reduction in pregnancy ketone levels, which prevents normal development of the decidua [75,76]. Wang et al. acquired methyl 3,4-dihydroxybenzoate from D. superbus, using an in vivo mouse (pregnancy) uterus spontaneous life record as a model [38]. The result indicated that 200 μg/kg methyl 3,4-dihydroxybenzoate excited the pregnant uterus, which was monitored by uterine smooth muscle contraction strength and amplitude [38]. Guo et al. used in vitro rat and in vivo rabbit uterus models to investigate the synergistic effects of the extracts and prostaglandin E2 [77]. The experimental results indicated that alcohol extracts excited uterine smooth muscles more when combined with the drug, and the degree of contraction in the treatment group (4.33 ± 2.19 cm) was significantly higher than that before administration. Therefore, the ethanol extract of D. superbus has an obvious excitatory effect on the uterus of anesthetized free in vivo rats and the isolated peroneal muscle strips of rats. These studies show that Dianthi herba can stimulate the uterus, which is consistent with its traditional use in China to treat amenorrhea.

Antimicrobial activity
The antimicrobial activity of various Chinese herbs against Chlamydia trachomatis in the urogenital tract was tested in a previous study, and the results showed that Qumai decoction exhibits high sensitivity and a minimum inhibitory concentration (MIC) of < 2 mg/mL. Furthermore, the volume and quantity of Chlamydia inclusions decreased gradually with increasing Qumai decoction concentration, and finally disappeared. Chlamydia trachomatis growth was found in the blank control pores, but not in the negative control wells [78]. Antimicrobial experiments with several strains were used to compare the plant-based cyclopolypeptide (XIII) from D. superbus with the standard drug, griseofulvin. Cyclopolypeptide (XIII) exhibited potent activity against the pathogenic fungus, Candida albicans, with an MIC of 6 µg/mL [79]. Moreover, the inhibitory effects of ethanol and water extracts of D. superbus on the activities of Shigella dysenteriae, Bacillus cereus, and Vibrio cholerae were studied. The MIC was 6.25-12.550 mg/mL, and the minimum bactericidal concentration (MBC) was 12.525-12.550 mg/mL. The results showed that the ethanol and water extracts of D. superbus had positive inhibitory effects on S. dysenteriae, B. cereus, and V. cholerae [10].
These studies show that Dianthi herba has antibacterial effects, but most of these studies have been carried out in vitro, and it is necessary to use modern technology to study the potential mechanism of action and to translate the in vitro and in vivo research findings into clinical applications.

Other effects
Yun et al. isolated two bioactive compounds from D. superbus, 4-hydroxy-benzeneacetic acid and 4-methoxybenzeneacetic acid, to determine the components mediating its neuroprotective activity against glutamateinduced death of hippocampal neuronal HT22 cells. The two compounds effectively protected HT22 cells against glutamate toxicity [42]. Glutamate is obviously toxic to hippocampal neurons and was used to induce damage in the model used to determine the effects of D. superbus on cell viability. The cell viability was evaluated using the MTT assay. The EtOAc soluble fraction of D. superbus extract was obviously active, and it increased cell viability to 73.89% and 94.35% at concentrations of 10 and 100 µg/ mL, respectively. These phenomena suggest that Dianthi herba may have neuroprotective activity.
Dianthi herba possesses several other biological activities such as hemolytic, anthelmintic, and antiallergic effects. In a previous study, a high concentration (100%) of D. superbus alcoholic extract showed slight hemolytic effects and insect-repellent activity at a dose of 2 mg/mL [80]. Yoon et al. studied the protective effect of EtOAc extract of D. superbus in renal inflammation and fibrosis; the extract at a dose of 10 or 50 mg/kg/day administered to db/db mice for 8 weeks significantly improved the blood glucose and insulin levels, insulin resistance, homeostasis model assessment index, and HbA1c level. Therefore, the extract can be used as a potential drug to treat glomerulonephritis and glomerulosclerosis, which lead to diabetic nephropathy [81]. Zhang et al. found that Gualou Qumai decoction reduced the level of C-reactive protein (CRP), IL-6, and TGFβ1 in the renal tissue of rats and exerted the therapeutic effect of alleviating inflammatory reaction and renal fibrosis. In a previous study, Dianthi herba combined with Magnolia officinalis cortex, Aurantii fructus immaturus, and other medicines was used to treat pancreatic hydatoncus diseases; the results showed considerable clinical curative effects of the combination [1]. The extract of D. chinensis (500 μg/ mL) inhibited the production of IgE in vitro and in vivo in peanut-allergic mice, considerably reduced the allergic reaction caused by peanuts, and exerted a certain antiallergic effect [83].
In general, Dianthi herba has various pharmacological activities; however, screening and evaluation of its active compounds remain at the crude extract level. Research on its chemical composition, biological activity, pharmacodynamics, and mechanism of action remain limited. These gaps should be addressed in the future.

Toxicity
Currently, there are only a few reports on the toxicity of Dianthi herba. The suggested daily dose of Dianthi herba is 9-15 g, and pregnant women should use it cautiously, as documented in the Pharmacopoeia of the PRC (Pharmacopoeia Commission of PRC, 2020). A study on the antifertility and genotoxic effects of Dianthi herba decoction in pregnant mice has shown that doses of 10, 15, and 30 g/kg considerably affected the implantation period and early pregnancy. Furthermore, 15 and 30 g/kg Dianthi herba exerted certain mid-term pregnancy-terminating effect, which was apparent in mice in the late pregnancy stage, where it shortened the gestation period and reduced the weight of offspring. This is consistent with the "abortion and inferior occlusive blood" effects of Dianthi herba recorded in Shennong's Classic Materia Medica. Dianthi herba caused abortion in mice in the early pregnancy stage, indicating the lethal effect of this drug [84,102]. In addition, Dianthi herba can cause damage to the kidney. Previously, different doses (0.25, 0.5, and 2.5 g/mL) were used to determine the serum biochemistry of liver and kidney function. Organ index analysis and histopathology were performed to detect the morphological changes of the main organs in mice. qPCR was used to determine the role of organic anion transporters OAT1 and OAT3 in the toxic injury in mice treated with Dianthi herba. The results showed that the middle and high dose groups of Dianthi herba had different degrees of damage of the main organs in mice, especially the kidney was severely damaged [103,104].

Clinical applications
Clinical and human studies have shown that Dianthi herba is mainly used in the treatment of urinary diseases, diabetic nephropathy, edema, cysts, esophageal tumors, and rectal cancer. In piglets and lamb with dysentery, Dianthi herba has a unique curative effect. In a recent study on chronic prostatitis, 60 patients with damp-heat, stasis type, chronic prostatitis were selected and randomly divided into two groups, with 30 patients in each group. The mean age of the control group was 31.1 years and the mean course of the disease was 33 months. A Gualou Qumai pill and Taohe Chengqi decoction were added and removed for 8 weeks, at one dose a day, and 400 mL of extract was extracted by water decoction and administered twice, in the morning and evening, after a meal. The results showed that there are 27 columns (90%) were effective in the 30-column Gualou Qumai pill treatment group, indicating that the Gualou Qumai pill and Taohe Chengqi decoction can alleviate the symptoms of chronic prostatitis [3].
In a previous study, 60 patients with diabetic nephropathy, aged 40-75 years, with a duration of diabetes ranging from 4 to 10 years, were treated with a TCM decoction of modified Gualou Qumai (decocted in water twice) for 1 month, at one dose a day. Three hundred milliliters of warm decoction was administered twice, in the morning and evening. The results showed that there are 25 columns (83.33%) were effective among the 30 columns of treatment groups, indicating that the Jiawei Gualou Qumai decoction can effectively improve the clinical symptoms of patients and assist in alleviating albuminuria and renal mesangial lesions, and delaying the process of diabetic nephropathy.
Dianthi herba is also effective in the treatment of urinary retention after an anorectal operation [85]. Recently, 60 patients with urinary retention after anorectal disease were selected and were administered Qin Yanning granules (20 g each time). The patients' first micturition time and voiding volume, inter alia, were measured. There are 55 (91.7%) patients was effective Among the 60 patients, indicating that Qin Yanning granules could significantly improve the micturition and abdominal distension symptoms of patients and improve the clinical effect [86]. Shoulin used Gualou Qumai pill to treat patients with edema, and the reexamination showed that the total protein and albumin level, 24-h urine volume, and liver function were substantially improved, indicating that the Gualou Qumai pill can effectively improve the clinical symptoms of the patients [87]. The network diagram of chemical composition, pharmacological action and traditional application of Dianthi herba is shown in Fig. 13.

Conclusion and perspective
The traditional use, chemical composition, and extensive pharmacological activities of Dianthi herba are summarized based on information obtained from traditional literature records and modern literature. Dianthi herba has a long history of medicinal use in China. After decades of modern research, the main components such as saponins, flavonoids, peptides, anthraquinones and volatile oil have been isolated and identified, and their pharmacological activities have also been verified. Furthermore, through pharmacological experiments, the traditional applications of Dianthi herba have been confirmed, including the treatment of urinary tract infection and dysmenorrhea. However, more in-depth studies on the complex pharmacological effects of the Dianthus spp. and a complete phytochemical overview are needed to clarify the mechanism of action of Dianthi herba.
In modern times, D. superbus var. longicalycinus, D. versicolor, D. amtifolia, D. chinensis var. liaotungensis, and D. shandongensis are used as main sources of various medicines that can be confused with each other, and this could seriously affect treatment outcomes. According to the Chinese Pharmacopoeia, the identification methods for Dianthi herba are limited to thin layer chromatography and microscopy techniques. Therefore, it is necessary to establish an efficient, accurate, and scientific identification method to ensure the authenticity of the product. In addition, Dianthi herba medicinal materials are mostly found in wild medicinal materials. To ensure the conservation of sources of Dianthi herba medicinal materials and reduce the confusion of products and substitutes, attention should be paid to develop large-scale cultivation techniques. Studies should also focus on the establishment of a unified standard system and quality grade standards.
Saponins and cyclic peptides are considered the main pharmacologically active components in several bioactive compounds identified in Dianthi herba, and also new compounds isolated from Dianthi herba. Basic research on the pharmacological activities of Dianthi herba is limited, focusing mainly on the activity of the extracted parts. Therefore, the study on the biological activity of other chemical components and the interaction and structureactivity relationship between saponins and cyclic peptides should be strengthened in the future. In addition, clinical studies should be conducted to effectively evaluate the efficacy, adverse reactions, and toxicity of Dianthi herba.
Currently, Dianthi herba is mainly composed of aboveground parts, and little is known about the composition and activity of the underground parts. Therefore, in-depth studies on other parts of the plant should be carried out in order to reveal its overall development potential. Finally, in addition to high efficacy and less adverse effects, a qualified drug should have good pharmacokinetic properties, but there are only a few studies on the pharmacokinetics of Dianthi herba, which greatly limits the development of the plant in the future market of traditional Chinese medicine.
Herein, we summarized the traditional applications, chemical composition, and pharmacological action of Dianthi herba globally. Further exploration is still needed to guarantee the rational utilization of Dianthi herba. Moreover, considerable attention should be paid to market demand and quality control.

Availability of data and materials
All data generated or analysed during this study are included in this published article [and its additional files].