Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography

Table 3 Recovery of seven markers in Gegen Tang sample*

Compounds	Contents (μg/g)	RSD% (n = 6)	Added (μg/mL)	Obtained (μg/mL)	Recovery (± RSD %, n = 3)
Puerarin	3920	0.61	3.50	3.54	101.20 ± 0.74
			8.75	8.68	99.26 ± 0.43
			13.85	13.75	99.41 ± 0.51
Paeoniflorin	3219	0.54	3.63	3.60	99.28 ± 1.61
			9.07	8.99	99.14 ± 1.07
			13.27	13.16	99.33 ± 0.51
Cinnamic acid	317	0.73	0.27	0.27	99.78 ± 1.01
			0.68	0.68	100.23 ± 1.67
			0.87	0.87	100.01 ± 1.06
Daidzain	283	1.14	0.19	0.19	100.35 ± 1.10
			0.48	0.48	98.94 ± 1.50
			0.78	0.77	98.76 ± 0.76
Glycyrrhizin	4138	0.55	3.56	3.59	100.92 ± 1.16
			8.89	8.93	100.54 ± 0.40
			16.96	17.09	100.73 ± 0.30
Ephedrine	4283	0.67	3.70	3.74	101.03 ± 0.83
			9.26	9.30	100.38 ± 1.34
			14.41	14.29	99.22 ± 1.35
Pseudoephedrine	1007	1.21	0.50	0.96	101.70 ± 1.17
			2.37	2.39	101.02 ± 2.01
			4.19	4.23	100.71 ± 0.72

ISSN: 1749-8546