Figure 4

Reduction of TG content by Radix Angelica Sinensis and Rhizoma Alismatis in differentiated 3T3-L1 adipocytes. A: Lipogenic differentiated 3T3-L1 cells were either serum-starved or treated with insulin (10 μg/ml) for three days, and then stained by Oil red O dye. The amount of stained TG (red color) was quantified at 490 nm absorbance. B: Lipogenic differentiated 3T3-L1 cells were treated with Rhizoma Alismatis and Radix Angelica Sinensis (0.1, 1 and 10 mg/ml) for 72 hours. The reduction of TG content was measured. Data are expressed as mean ± SD of the percentage of control (i.e. water-treated control set as 100) and number of independent experiments (n) = 5; P < 0.01 (**); P < 0.001 (***).