| Â | PHY906-6 | PHY906-7 | PHY906-8 | HQT-E | HQT-F |
|---|
PHY906-6 | 1 | 0.99 | 0.98 | 0.94 | 0.68 |
PHY906-7 | Â | 1 | 0.97 | 0.92 | 0.71 |
PHY906-8 | Â | Â | 1 | 0.97 | 0.61 |
HQT-E | Â | Â | Â | 1 | 0.58 |
HQT-F | Â | Â | Â | Â | 1 |
- Five batches were selected from the total of 17 batches, based on chemical fingerprint similarity. Three batches from Group I (PHY906-6, PHY906-7, PHY906-8) that exhibit high similarity were chosen. Two batches, HQT-E and HQT-F from Group II were selected that were manufactured by the same vendor. HQT-E exhibited the highest similarity and HQT-F exhibited the lowest chemical fingerprint similarity to PHY906-6. Differential gene expression values for the 15 gene bioresponse fingerprint were measured by qRT-PCR using Assay-on-Demand from Applied Biosystems, standardized HepG2 cells and a one IC50 dose level of the PHY906 or the HQT batch. PSI values are based on the Pearson correlation between the ratio matrices of the 15 differential gene expression changes and vary between 0.0 (no similarity) and 1.0 (complete identity). The gene expression values ranged between -2.4 and 405 and are shown in supplemental table 3 for PHY906-6. Duplicate experiments with either PHY906-6 or HQT-F results in PSI values of 0.97 indicating a high level of reproducibility. Comparison of PSI values using this 15 gene fingerprint indicate high similarity (0.92-0.99) between batches PHY906-6, PHY906-7, PHY906-8 and HQT-E, but significantly lower similarity (0.58-0.70) with batch HQT-F.
