Figure 4

Extract attenuates the induction of ER stress. (A) Extract prevented the increase of thapsigargin-stimulated luciferase activity in thapsigargin-treated HepG2. HepG2 cells were transfected with ERSE-Luc reporter and then treated with extract (5 μg/ml), rosiglitazone (10 μM), or macelignan (10 μM) for 24 hours in the presence of thapsigargin (10 ng/ml). (B) Extract increased the levels of GRP78 and peIF. HepG2 cells were preincubated with extract (5 μg/ml), rosiglitazone (10 μM), or macelignan (10 μM) for 24 hours and then treated with thapsigargin (10 ng/ml) for 24 hours. GRP78 p-eIF were measured by Western blot analysis. Data represent are shown as mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)