Figure 3

Western blot analysis of CYP2E1 levels in Chang cell line. The expression level of CYP2E1 protein was detected by western blot analysis with anti-human CYP2E1 antibody. Chang cells were treated with saline (lane 1, untreated), CCl₄ (lane 2, control), CCl₄ + silymarin 1 mg/mL (lane 3), CCl₄ + HCIF 1 mg/mL (lane 4), CCl₄ + HCIF 2 mg/mL (lane 5) and CCl₄ + HCIF 4 mg/mL (lane 6). All bands were quantified by densitometric analysis. Each bar represents the mean ± SD replicated in triplicate. ^**P < 0.01 and ^***P < 0.001, significantly different from the control group. ^#P < 0.001, significantly different from the untreated group.