Fig. 8

The effects of PNS on oxidative stress and glucose level in zebrafish. A PNS compound target proteins regulate anti-oxidants through TXNRD1 enzyme and transcription factors including NRF2, p53, AP-1 and NF-kB. Hmox1, SOD1, GCLc and PRDX1, takes place in the ROS suppression pathway and inhibit reactive oxygen radicals and hydrogen peroxide. Green rectangles are predicted PNS compound target proteins, purple rectangles are enzymes, orange rectangles are transcription factors and red rectangles are DEGs from our RNA-seq data. B Fluorescence value of ROS in WT zebrafish post PNS treatment. C Glucose and D ROS levels in high-sugar model zebrafish after PNS treatment. E Real-time qPCR results for selected genes involved in oxidative stress, insulin regulation and fatty acid metabolism. **p < 0.01; ***p < 0.001