Table 1 Sequences of primer pairs of mRNA and miRNA used in the real-time quantitative PCR reaction
Gene symbol | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
Hmox1 | ACCGTGGCAGTGGGAATTTA | CAAGATTCTCCCCTGCAGAGA |
GCLc | TGAGTCTCCTCTTGCTGTGTACGT | GGGACTGCTCTGCAAAGGAA |
Txn1 | GTGGTGTGGACCTTGCAAAA | CTGGCAGTCATCCACGTCTACTT |
SOD1 | CACTGCAGGACCTCATTTTAATCC | GTCCTTTCCAGCAGCCACAT |
PRDX1 | AGCTCGACTCTGCTGATAGCAA | AATTGTCCATCGGGCATAACA |
Lpl | GCTCCATCCATCTCTTCATTGAC | AGGCAGAGCCCTTTCTCAAAT |
ABCG1 | TGGAGAACGCGAAGCTATACC | CAGCCCGGATTTTGTATCTCA |
Cav-1 | CCATGGCAGACGAGGTGAAT | ACCACGTCGTCGTTGAGATG |
RPS6 | GAAAGCCCTTAAACAAAGAAGGTAAG | ATACGTCGGCGTTTGTGTTG |
Gab1 | TCCCACCACACCCAGACACT | TGGGCTCTGGTGGGTTCA |
GAPDH | GGTGGACCTCATGGCCTACA | CAGCAACTGAGGGCCTCTCT |
MiRNA | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
Rno-miRNA-139-5p | GTCTACAGTGCACGTGTC | CCAGTTTTTTTTTTTTTTTCTGGAG |
Rno-miRNA-24-3p | AGTGGCTCAGTTCAGCA | CCAGTTTTTTTTTTTTTTTCTGTTCCT |
Rno-miRNA-671 | GCAGTCCGGTTCTCAG | GGTCCAGTTTTTTTTTTTTTTTGGT |