Fig. 2

Formononetin protected the integrity of the intestinal barrier and reduced bacterial translocation in AP. C57BL/6J mice were pretreated with formononetin for 1 week and underwent induction of AP. A, B Representative H&E staining of the colon (A) and histopathology score (B); scale bar, 50 μm. C-F qRT-PCR for colonic mRNA quantitative analysis of tnf-α (C), mcp-1 (D), il-6 (E) and il-1β (F). G-I qRT-PCR for colonic mRNA quantitative analysis of zo-2 (G), occludin (H) and claudin-1(I). Western blot and quantitative analysis of colonic ZO-2, Occludin and Claudin-1 (J). K, L qRT-PCR for mRNA relative content of E. coli in colon and pancreas. M Measurement of serum endotoxin. Data were expressed as mean ± SD, n = 3–8 in each group. * p < 0.05, ** p < 0.01, *** p < 0.001