Fig. 5

Molecular docking analysis of formononetin and the keap1 protein. Formononetin bound to the keap1 (PDB ID: 5CGJ) in the most stable pose and binding motifs was depicted with several high affinity interactions between formononetin and the keap1 pocket. Formononetin is shown as green sticks in the keap1 pocket A, B. Bonds are shown as dashed lines color-coded as follows: hydrogen bonds in blue, and hydrophobic interactions in gray C. ALA alanine, VAL valine, ILE isoleucine. D Co-immunoprecipitation analysis of the interaction between Keap1 and Nrf2 in Colonic tissues