Fig. 1

Inhibitory profiles of glabridin for platelet aggregation and Lyn, Fyn, Syk and integrin β₃ activation stimulated by collagen in human platelets. A Chemical structures of glabridin (C₂₀H₂₀O₄). B Washed human platelets (3.6 × 10⁸ cells/mL) were preincubated with a solvent control (0.1% DMSO) or glabridin (5–30 µM), followed by the addition of collagen (1 µg/mL) to stimulate platelet aggregation. For further experiments, washed platelets were preincubated with a solvent control (0.1% DMSO) or glabridin (20 and 30 µM), followed by the addition of collagen (1 µg/mL) to stimulate C Lyn, D Fyn, E Syk, and F integrin β₃ phosphorylation. Data are presented as mean ± standard error of the mean (n = 4). ***P < 0.001, compared with the resting control (Tyrode’s solution); ^##P < 0.01 and ^###P < 0.001, compared with the 0.1% DMSO-treated group