Fig. 3

Effects of glabridin, BAY11-7082, and Ro106-9920 in regulating collagen-stimulated NF-κB activation measured using confocal laser fluorescence. Washed platelets were preincubated with 0.1% DMSO, glabridin (30 µM), BAY11-7082 (10 µM), or Ro106-9920 (10 µM), followed by the addition of collagen (1 µg/mL) to trigger platelet activation. The confocal images of A, B IKK (green fluorescence), C, D p-p65 (green fluorescence), and α-tubulin (blue fluorescence) were observed using goat anti-rabbit CF^TM488A and goat anti-mouse CF^TM405M dyes, respectively, as described in “Materials and methods”. The confocal images represent four similar experiments. bar: 2.5 μm. The intensity of green fluorescence representing the A, B phospho-IKK and C, D phospho-p65 were quantified in at least four different fields per view (mean fluorescence intensity [MFI]). Data are presented as mean ± standard error of the mean. ***P < 0.001, compared with the resting control (Tyrode’s solution); ^###P < 0.001, compared with the 0.1% DMSO-treated group