Fig. 4
Inhibitory activity of BAY11-7082 in Lyn, Fyn, Syk, integrin β3, phospholipase Cγ2, and protein kinase C activation in human platelets. A Washed platelets were preincubated with a solvent control (0.1% DMSO) or BAY11-7082 (5 and 10 µM), followed by the addition of collagen (1 µg/mL) to stimulate A Lyn, B Fyn, C Syk, (D) integrin β3, E phospholipase Cγ2 (PLCγ2), and F protein kinase C (PKC) activation. Data are presented as mean ± standard error of the mean (n = 4). ***P < 0.001, compared with the resting control (Tyrode’s solution); #P < 0.05, ##P < 0.01, and ###P < 0.001, compared with the 0.1% DMSO-treated group