Fig. 5
Effects of glabridin and BAY11-7082 in regulating Fyn, Syk, phospholipase Cγ2, and integrin β3 phosphorylation in platelet activation measured using confocal laser fluorescence. Washed platelets were preincubated with 0.1% DMSO, glabridin (30 µM), or BAY11-7082 (10 µM), followed by the addition of collagen (1 µg/mL) to trigger platelet activation. The confocal images with green fluorescence of A, B Fyn, C, D Syk, E, F phospholipase Cγ2 (PLCγ2), and G, H integrin β3 phosphorylation and blue fluorescence of α-tubulin were produced using goat anti-rabbit CFTM488A and goat anti-mouse CFTM405M dyes, respectively. The confocal images represent four similar experiments. bar: 2.5 μm. The intensity of green fluorescence representing the phosphorylation of A, B Fyn, C, D Syk, E, F PLCγ2 and G, H integrin β3 were quantified in at least four different fields per view (mean fluorescence intensity [MFI]). Data are presented as mean ± standard error of the mean. ***P < 0.001, compared with the resting control (Tyrode’s solution); ###P < 0.001, compared with the 0.1% DMSO-treated group