Fig. 6

Effectiveness of glabridin on thromboembolism in lungs and platelet plug occlusion in mesenteric venules of mice. A To induce acute pulmonary thrombosis, 0.1% DMSO or glabridin (6 and 12 mg/kg) was intraperitoneally administered in mice, and ADP (700 mg/kg) was injected into the tail vein. Histological examination of pulmonary thrombosis (black arrows) was undertaken by staining lung tissue sections with hematoxylin–eosin as described in “Materials and methods”. B Mice were administered an intraperitoneal injection of solvent control (0.1% DMSO) or glabridin (6 and 12 mg/kg), and mesenteric venules were irradiated using fluorescein to induce microthrombus formation (occlusion time). Microscopic images were also recorded at 5 and 150 s after irradiation. Black arrows indicate platelet plug formation (×40 magnification). Data are presented as the mean ± standard error of the mean (n = 12)