Fig. 3

DHJST reduces cartilage degradation in KOA mice C57BL/6 mice were injected with 4% papain 0.1 ml (once a week for three weeks) into the right knee joint to induce KOA. After that, they were treated with 2 g/kg or 4 g/kg DHJST for 4 weeks. A, B Immunohistochemical staining of MMP2 in the right knee cartilage of mice and its mean optical density analysis, N = 6. C, D Western blot detection of collagen 2 and collagen 4 in the right knee cartilage of mice and the analysis of their relative expression levels, N = 4. E, F Immunohistochemical staining of MMP2 in the synovium of the right knee joint of mice and its mean optical density analysis, N = 6