Fig. 6

NLRP3 interference did not affect the expression level of notch1 or downstream transcriptional signals in the synovia of KOA mice C57BL/6 mice were injected with control AAV2 (Con AAV) or NLRP3 shRNA AAV2 (nlr_KD AAV) into the tail vein for 3 weeks. Then, 4% papain 0.1 ml (once a week for three weeks) was injected into the right knee joint of the mice to induce KOA, and 4 g/kg DHJST was used to treat the mice for 4 weeks. A, B Immunohistochemical staining of Notch1 in the synovium of the right knee joint of the mouse and its mean optical density analysis C, D Immunohistochemical staining of NLRP3 in the synovium of the right knee joint of the mouse and its mean optical density analysis. (E-F) mRNA expression levels of HES1 and HEY1 in the synovium of the right knee joint of mice. N = 6 in A–D, N = 8 in E–F