Fig. 7

Notch1 overexpression abolishes the inhibitory effect of DHJST on cartilage degradation in KOA mice C57BL/6 mice were injected with control AAV2 (Con AAV) or Notch1-overexpressing AAV2 (notch1_OE AAV) into the tail vein for 3 weeks. Then, 4% papain 0.1 ml (once a week for three weeks) was injected into the right knee joint of the mice to induce KOA, and the mice were treated with 4 g/kg DHJST for 4 weeks. A, B Immunohistochemical staining of Notch1 in the right knee joint cartilage of the mice and its mean optical density analysis, N = 6. C, D Immunohistochemical staining of Notch1 in the synovial membrane of the right knee joint of the mice and its mean optical density analysis, N = 6. E, F mRNA expression levels of HES1 and HEY1 in the synovium of the right knee joint of mice, N = 8. G, H Immunohistochemical staining of MMP2 in the synovium of the right knee joint of mice and its mean optical density analysis, N = 6. I, J Immunohistochemical staining of MMP2 in the cartilage of the right knee joint of mice and mean optical density analysis, N = 6. K, L Western blot detection of collagen 2 and collagen 4 in the cartilage of the right knee joint of mice and their relative expression levels, N = 4