Fig. 8

Notch1 overexpression abolishes the inhibitory effect of DHJST on synovial NLRP3 activation in KOA mice C57BL/6 mice were injected with control AAV2 (Con AAV) or Notch1-overexpressing AAV2 (notch1_OE AAV) into the tail vein for 3 weeks. Then, 4% papain 0.1 ml (once a week for 3 weeks) was injected into the right knee joint of the mice to induce KOA, and 4 g/kg DHJST was used to treat the mice for four weeks. A A representative picture of the morphology of the right knee joint of the mice after treatment. B Line plots recorded the thickness of the right paw of the mice before modeling (baseline), after modeling (modeling), and after weekly administration (1–4 weeks). Con AAV groups vs. notch1_OE AAV groups, & P < 0.05; DHJST groups vs. Model groups, * P < 0.05. C Serum IL-1β levels in mice were detected by ELISA. (D-E) Immunohistochemical staining of NLRP3 in the synovium of the right knee joint of mice and mean optical density analysis. F, G mRNA expression levels of Caspase3 and IL-1β in the synovium of the right knee joint of mice. N = 6 in D, E; N = 8 in A–C and F, G.