Fig. 2

Aucubin reduced VRI-induced vascular insufficiency in zebrafish. A 24 hpf Tg(fli-1a:EGFP)^y1 zebrafish embryos were pre-treated with 500 ng/mL VRI for 3 h, rinsed, and immersed in various concentrations of aucubin (5–20 µM) for 24 h. The expression of EGFP in ISVs of zebrafish embryos. B Quantitative analysis showing the concentration-dependent effects of aucubin on the recovery of ISVs. Data are presented as means ± SD for three independent trials. ^##p < 0.01 versus the control group; **p < 0.01 versus the group treated with VRI alone