Fig. 3

Effects of aucubin on VRI-induced down regulation of several pro-angiogenic factors mRNA expression in zebrafish. Total RNAs from different groups of zebrafish pre-treated with VRI (500 ng/mL) for 3 h, and then treated with different concentrations of Aucubin (5, 10 and 20 μM) after VRI withdrawal, were isolated and reverse transcribed to cDNA. The mRNA levels of flt-1 (A), kdr (B), kdrl (C), vegfaa (D), ang-1 (E), ang-2 (F), tie1 (G) and tie2 (H) were tested by RT-PCR. Data are expressed as the mean ± SD, ^##p < 0.01 versus the control group; *p < 0.05 and **p < 0.01 versus the group treated with VRI alone