Fig. 5

Effects of aucubin on the expression of proteins associated with the regulation of angiogenesis. HUVECs were starved in low serum media (0.5% FBS) for 3 h, then treated with VEGF (50 ng/mL, positive control) and different concentrations of aucubin (0–50 µM) for 2 h. A Several key proteins associated with signaling pathways of proliferation and survival in angiogenesis were determined by Western blotting. B–G Expression levels of p-Akt, p-MEK1/2, p-ERK1/2, p-mTOR, p-FAK and p-Src, respectively. Data are presented as the percentage of the control group (mean ± SD of three independent trials). *p < 0.05 and **p < 0.01 versus the control group