Table 1 Primers used for quantitative RT-PCR
Primer name | Sequence |
|---|---|
Flt-1 forward | 5′-AACTCACAGACCAGTGAACAAGATC-3′ |
Flt-1 reverse | 5′-GCCCTGTAACGTGTGCACTAAA-3′ |
kdr forward | 5′-CAAGTAACTCGTTTTCTCAACCTAAGC-3′ |
kdr reverse | 5′- GGTCTGCTACACAACGCATTATAAC-3′ |
kdrl forward | 5′-GACCATAAAACAAGTGAGGCAGAAG-3′ |
kdrl reverse | 5′-CTCCTGGTTTGACAGAGCGATA-3′ |
Ang-1 forward | 5′-ACATGCAAGTGTGCACTGATGCTC-3′ |
Ang-1 reverse | 5′-TTCCGACATGCTGTCCTTGTCTGT-3′ |
Ang-2 forward | 5′-CCAATCTTCTAAGCCAATCAGCGGAA-3′ |
Ang-2 reverse | 5′-CCACATCTGTCAGTTTGCGCGTGTTT-3′ |
Tie-1 forward | 5′-AGAGGCACGGAAGGCTTATG-3′ |
Tie-1 reverse | 5′-TAGCCTCCCTTGGGCTATGA-3′ |
Tie-2 forward | 5′-TGAGCTACCTGAGCCAGAAACAGT-3′ |
Tie-2 reverse | 5′-TCTTCGCCACAAAGTTCTCTCCCA-3′ |
Vegfaa forward | 5′-TGTAATGATGAGGCGCTCGAA-3′ |
Vegfaa reverse | 5′-AGGCTCACAGTGGTTTTCTT-3′ |