Fig. 4From: Research on the interaction of astragaloside IV and calycosin in Astragalus membranaceus with HMGB1Fluorescence spectrum of HMGB1 in the present of astragaloside IV (A) and calycosin (B), (C) Concentration-dependent quenching on the fluorescence intensity of HMGB1 induced by two ligand molecules. (The concentration of HMGB1 was 3 µM and that of astragaloside IV, calycosin (A-I) were 0, 2.5, 5, 10, 20, 40, 60, 80, 100 µM, λex = 273 nm)Back to article page