Fig. 1

GQD alleviates anxiety-like behaviors and improved gut microenvironment in Meth-withdrawn mice. A The experimental timeline settings in wild-type (WT) mice. B Anxiety-like behavior tests, including elevated plus maze test (EPM) and open field test (OFT). Pictures to the right show the representative traces of the corresponding behavioral tests. C The H&E staining pictures of colon mucosa. Scale bar: 50 μm. D The colon permeability test with FITC-Dextran 4000da detection. E The staining densities of Claudin-3 or ZO-1 in colon mucosa. Scale bar: 50 μm. F Western blotting of Claudin-3 or ZO-1 protein levels in colon. G Western blotting of several pro-inflammatory factors, including TNFα, IL-6 and IL-1β, and the anti-inflammatory factor IL-10 in colon. OA: open arms of EMP. CA: central area of OFT. Sal: saline withdrawal mice. Meth: Meth-withdrawn mice. V: vehicle-treated mice. GQD: GQD-treated mice. N.S., P > 0.05; *, P < 0.05; **, P < 0.01 versus the indicated controls