Fig. 10

COL12A1 inhibits GC cell ferroptosis. A–E COL12A1 knockdown promoted GC cell ferroptosis. Ferroptosis was evaluated by detecting ROS, MDA, Fe²⁺ and GSH levels. This work acquired confocal laser scanning microscope images for ROS production. F Low-expression of COL12A1 impaired the shape of mitochondrial cristae (white arrow) and promoted the density of mitochondrial membrane (black arrow) in the ferroptosis process of MKN-45 and SGC-7901 cells. This work obtained TEM images for mitochondrial morphology. G, H The qRT-PCR and WB assays were carried out to detect the transfected MKN-45 and SGC-7901 cells for assessing ferroptosis markers level. **P < 0.01 sh-COL12A1 group vs the control group. Scale bar = 400 μm (E). Scale bar = 1 μm (F)