Fig. 11
JYQHD inhibits the stemness and induces ferroptosis of GC cells via COL12A1. A, B COL12A1-plasmids were transfected into MKN-45 and SGC-7901 cells, qRT-PCR and WB assays were conducted to confirm COL12A1 over-expression efficiency. C JYQHD-medicated serum was added to treat transfected MKN-45 and SGC-7901 cells, and sphere-formation assay was conducted to analyze the sphere size and number. D–H Those transfected MKN-45 and SGC-7901 cells were exposed to JYQHD-medicated serum, and ferroptosis was evaluated by detecting Fe2+, MDA, GSH and ROS levels. I, J The qRT-PCR and WB assays were carried out to detect the pretreated MKN-45 and SGC-7901 cells for assessing ferroptosis and stemness related markers expression. **P < 0.01 JYQHD group vs the control group. ##P < 0.01 JYQHD + oe-COL12A1 group vs JYQHD group. Scale bar = 400 μm (C). Scale bar = 400 μm (H)