Fig. 2

JYQHD inhibits the growth of GC cell lines. A Both JYQHD-medicated serum (100%) and 5-FU were used to treat MKN-45 and SGC-7901 cells for 48 h, and the viability of GC cells was inhibited in both groups. B JYQHD-medicated serum at diverse contents was used to treat MKN-45 and SGC-7901 cells for a 24 h period, and inhibition rate was determined through CCK8 assay. A 10% concentration of JYQHD-medicated serum was defined as an IC50 value. C JYQHD-medicated serum suppressed stemness of GC cells. 10% concentration of JYQHD-medicated serum was added to treat MKN-45 and SGC-7901 cells, and the size and number of sphere was measured by sphere-formation assay. **P < 0.01 JYQHD group or 5-FU group vs the control group. Scale bar = 400 μm (C)