Fig. 4
JYQHD inhibits the stemness of GC cells through the ferroptosis pathway. A–F JYQHD-medicated serum with/without ferroptosis signaling inhibitor Fer-1, and Fer-1 alone were added to treat MKN-45 and SGC-7901 cells. Cancer cell stemness was evaluated by sphere formation assay. Ferroptosis was evaluated by detecting Fe2+, MDA, GSH and ROS levels. G, H The qRT-PCR and WB assays were conducted to detect the pretreated MKN-45 and SGC-7901 cells for assessing stemness and ferroptosis-related markers. **P < 0.01 JYQHD group vs the control group. ##P < 0.01 JYQHD + Fer-1 group vs JYQHD group. Scale bar = 400 μm (A). Scale bar = 400 μm (F)