Fig. 8

COL12A1 enhances the stemness of GC cells. A, B MKN-45 and SGC-7901 cells were subject to COL12A1 shRNAs transfection. qRT-PCR and WB assays were conducted to verify the knockdown efficiency of COL12A1. C After transfected with COL12A1 shRNAs, GC cell activity was significantly decreased in sh-COL12A1 group. D COL12A1 knockdown suppressed the sphere size and number, as evidenced by sphere-formation assay. E, F The qRT-PCR and WB assays were conducted to detect the transfected MKN-45 and SGC-7901 cells for assessing stemness markers level. **P < 0.01 sh-COL12A1 group vs sh-NC group. Scale bar = 400 μm (D)