Fig. 4

Specific blocking NLRP3 inflammasome activation ameliorates mechanical allodynia of gout arthritis model mice. A Molecular structure of MCC950. B Schedual illustrating model establishment, MCC950 administration, PWT test and tissue collection. C–E Western blotting examination of protein expression of NLRP3 inflammasome components, including NLRP3 (C), Caspase-1 (D) and IL-1β (E) in ankle joints from control, MSU + Veh and MSU + MCC950 groups of mice. *p < 0.05, **p < 0.01. F Time courses showing 50% PWT changes of ipsilateral hindpaws. **p < 0.01 vs. control group. ^##p < 0.01 vs. MSU + Veh group. G Normalized AUC analysis of the data in panel F. *p < 0.05, **p < 0.01. n = 5–7 mice/group