Fig. 9
MCC950 or EA intervention reduces pain-sensing TRPV1 channel overexpression in DRG neurons innervating the hindlimbs. A Schedule depicting MCC950/vehicle, EA/sham interventions and DRG tissue collection. B Representative photos of TRPV1 immunostaining (in green) in DRG neurons across all groups. NeuN staining (in red) was used to identify all DRG neurons. C, D Summary of the percentage of TRPV1 positively stained (TRPV1+) neurons among all DRG neurons in each group. n = 5 mice/group. E Representative overlaid Ca2+ transients recorded in DRG neurons from control (left panel), MSU + shamEA (middle panel) and MSU + EA (right panel) group of mice. Capsaicin (300 nM) was applied to activate TRPV1 channels. KCl (40 mM) was perfused to determine all alive neurons. 90 cells were included in each panel. F Summarized data for delta increase in peak R340/380 caused by capsaicin of 3 groups. n = 5–6 tests/group. G Summarized data for the percentage of capsaicin positively responding (capsaicin+) neurons of 3 groups. > 200 neurons were included in each group. *p < 0.05, **p < 0.01