Fig. 6

Targeted accumulation of Em, EmB, and EmB-MO in the inflammatory colon. A Fluorescence in the heart, liver, spleen, lung, kidney, stomach, small intestine, and colon of normal mice and ulcerative colitis mice 12 h after the oral administration of cy7-Em, cy7-EmB, and cy7-EmB-MO. B Fluorescence intensity in each group. C Representative micrographs of H&E staining in the heart, liver, spleen, lung, and kidney for the evaluation of drug toxicology at animal level. Data represent the mean ± standard error of the mean (n = 3), ^***P < 0.001 vs. control group. Em: Emodin, 8 mg/kg/d; EmB-H: Emodin-borate nanoparticles, 6 mg/kg/d; and EmB-MO-H: EmB modified with oligomeric mannitol into microgels, 6 mg/kg/d