Fig. 5
FSM rescues the metabolic state induced by BaCl2 of the zebrafish embryos. A PCA scores plot (component 1 and component 2) for biomarkers selection between control group and model group. B Unsupervised hierarchical clustering dendrogram by Euclidean distance for groups’ separation analysis. C Volcano plot of differentially metabolites between control group and model group (model/control) using an adjusted p-value < 0.05 and |Log2FC |> 1 as cut off. D PLS-DA variable importance in projection (VIP) score plot with the top 15 important features ranked by their PLS-DA VIP scores. The colored boxes on the right represent the relative concentrations of the corresponding metabolite in each group. E PCA comparison for treatment effects trends of FSM among the six groups. The clustered colored dots represent the samples from each group. F Pathway analysis of FSM therapeutic markers. Annotated bubbles represent the important pathways from combined metabolite markers (194 markers, adjusted p-value (Holm p) < 0.05 and Impact > 0.1), negative ion (NI) metabolite markers (172 markers, Holm p < 0.05), and positive ion (PI) metabolite markers (44 markers, Holm p < 0.05)