Fig. 6
Effect of FSM from arrhythmia zebrafish exposure to BaCl2 and therapeutic markers (targets and metabolites) verification. A FSM Joint pathways analysis for FSM therapeutic targets and metabolomes. Annotated bubbles represent the important joint pathways using Holm p < 0.05 and Impact > 0.2 as cut off. Violin plots of (B) the adrenaline peak area and C the cAMP peak area of the QTOF-MS/MS spectra for selected FSM therapeutic metabolites in located pathways. Quantified FSM therapeutic biomarkers levels of D the adrenaline and E the cAMP (n = 6, mean ± s.d.). F qRT-PCR verification of FSM therapeutic target RYR2 mRNA expression (n = 3, mean ± s.e.m.). The results of the statistical analysis are presented in bar charts.*p < 0.05, **p < 0.005 compared with control gruop; #p < 0.05, ##p < 0.005 compared with model group. NS, not significant. G FSM exerts a significant rescue effect on BaCl2 induced arrhythmia characterized by elevated RYR2, adrenaline and cAMP in zebrafish embryos