Integrating animal experiments, mass spectrometry and network-based approach to reveal the sleep-improving effects of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture

Table 1 Differential potential metabolites characterized in mice brains and change trends

Mode	No	Metabolite	Formula	m/z	RT (min)	ZSSG vs ZSS		ZSSG vs GABA
Mode	No	Metabolite	Formula	m/z	RT (min)	Fold change	Trend	Fold change	Trend
ESI⁻	1	Aspartate	C₄H₇NO₄	132.03	0.43	0.79	↓*	0.82	↓
	2	Inosine	C₁₀H₁₂N₄O₅	267.07	0.46	1.13	↑*	1.13	↑*
	3	Phenyllactic acid	C₉H₁₀O₃	165.05	4.67	0.69	↓	0.49	↓*
	4	Uridine	C₉H₁₂N₂O₆	289.07	7.29	0.70	↓*	0.68	↓*
ESI⁺	5 6	2-monoolein 4-hydroxymethyl-3-methoxyphenoxyacetic acid	C₂₁H₄₀O₄ C₁₀H₁₂O₅	379.28 213.07	12.92 10.53	0.77 0.83	↓ ↓*	0.66 0.75	↓* ↓*
	7	Cis-gondoic acid	C₂₀H₃₈O₂	328.32	11.86	0.61	↓*	0.58	↓*
	8	3-Hydroxydecanoyl carnitine	C₁₇H₃₃NO₅	349.27	12.71	0.73	↓*	0.53	↓*
	9	Trans-2-hydroxycinnamic acid	C₉H₈O₃	165.05	0.81	0.41	↓*	0.38	↓*
	10	Oxidized glutathione	C₂₀H₃₂N₆O₁₂S₂	613.16	0.82	2.15	↑*	1.32	↑

ISSN: 1749-8546