Fig. 7

The effects of dendrobine on oxidative stress and autophagy are mediated by Rim15. A, B Dendrobine failed to decrease the levels of ROS (A) and MDA (B) in Δrim15 yeast mutant with K6001 background. C Fluorescence images of autophagy in K6001 yeast and Δrim15 yeast mutant with K6001 background after RES or dendrobine treatment. D Quantification result of C. The experiment was repeated three times and data from each experiment are displayed as mean ± SEM. Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test were utilized to evaluate statistically significant differences among groups. Two-tailed, unpaired, Student’s t-tests were used for comparison between two groups. ^*, ^** represent significant difference compared with negative control (p < 0.05, p < 0.01). ^#, ^##, ^### represent significant difference between K6001 yeast and Δrim15 yeast mutant with K6001 background upon corresponding treatment (p < 0.05, p < 0.01, p < 0.001)