Fig. 6

The regulation of the autophagic flux by NSC-EVs depends on exosomes . A Numbers of NSC-derived EVs obtained from NSCs pre-treated with PBS, high dose of LBP(H-LBP-EV), and high dose of LBP followed by treatment with the exosome secretion inhibitor GW4869 (H-NSC-EV + GW) were quantified by NTA. B Cell viability was examined in OGD-exposed primary neurons treated with PBS, NSC-EV, H-LBP -EV, and H-NSC-EV + GW. Cells incubated under standard cell culture conditions (Normoxia) were defined as 100% cell survival. C p-AMPK, p-mTOR, and LC3II were quantified by Western blotting in OGD-exposed primary neurons co-incubated with PBS, H-NSC-EV, and H-NSC-EV + GW. (D–F Quantitative analysis of p-AMPK, p-mTOR, and LC3II is shown. Data are presented as mean ± SD. Data are statistically different from each other with *P < 0.05, **P < 0.01, and ***P < 0.001