Fig. 7

NSC-EVs regulate autophagy and induce neuroprotection by miR-133a-3p . A Real-time Quantitative Polymerase chain reaction (qRT-PCR) quantification of miR-133a-3p and miR-206 concentrations in EVs obtained from NSCs pretreated with or without LBP as stated in the materials and methods section. B–C Cell apoptosis was examined by flow cytometry in primary neurons exposed to OGD that were treated with PBS, EVs obtained from normal NSCs, EVs isolated from high doses of LBP pretreated NSCs that were pretreated with scramble (H-NSC-EV^NC) or with anti-miR-133a-3p (H-NSC-EV^{anti−miR−133a}). Data are presented as mean ± SD. Neurons were cultured under standard conditions as a control. F Representative fluorescent images of OGD-exposed neurons in the treatment group aforementioned (PBS, NSC-EV, H-NSC-EV^NC, and H-NSC-EV^{anti−miR−133a}). G The density of NeuN⁺ cells were quantitatively analyzed. Data are statistically different from each other with *P < 0.05, **P < 0.01, and ***P < 0.001