Fig. 7

The AKT (Ser473)/ Nrf2 pathway mediates cyasterone against cell injury in MLE12 cells. A–I MLE12 cells were treated with ML385 (3 μM) or LY294002 (10 μM) for 2 h following incubation with cyasterone (100 μM) for 24 h with or without LPS (100 ng/ml) for 12 h. A–C The ROS content was analyzed by fluorescence microscopy and flow cytometry. D MitoSOX (5 μM) was measured by fluorescence microscopy. E–G Mitochondrial membrane potential (ΔΨm, MMP) was measured by flow cytometry and fluorescence microscopy with 5 μM JC-I. H, I The apoptosis of MLE12 cells was detected by flow cytometry. Data are expressed as mean ± SD, n = 3, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001