Fig. 5

Sal B promoted the autophagy of H9c2 induced by glucose deprivation. A Electron microscopy showed Sal B promoted the formation of autophagosome (Scale bar = 0.2 μm, n = 3); B Immunofluorescence staining showed Sal B increased the expression of LC3 (20×, n = 3); C, D WB showed Sal B regulated the expressions of LC3, Beclin1, Atg5 and P62 (n = 3); Data are shown as the mean ± SD. *P < 0.05, **P < 0.01, compared to GD 24 h without Sal B group