Fig. 1

Effects of mangiferin (MGF) and T0901317 (T0) on inhibition of AS progression. Apoe^−/− mice fed with high fat diet (HFD) in four groups (n = 10/group) received the following treatment for 16 weeks. Ctrl: vehicle; T0: oral gavage of T0 (1 mg∙kg⁻¹ bodyweight/day); MGF: oral gavage of MGF (200 mg kg⁻¹ bodyweight/day); T0 + MGF: oral gavage of T0 and MGF. After treatment, aortas were collected for the following assays. A Lesions in en face aorta were determined by Oil Red O staining and lesion areas were expressed as % of total en face aorta areas. Scale bar, 5 mm. ^***p < 0.001, ^**p < 0.01, ^*p < 0.05, NS: not significant (n = 10). B Sinus lesions in aortic root were determined by Oil Red O staining and lesion areas were expressed as μm²/section. Scale bar, 400 μm. ^*p < 0.05 (n = 9). C Collagen content areas were determined by Masson staining of aortic root cross sections and quantified by a computer-assisted image analysis protocol (n = 3). Scale bar, 100 μm. D Expression of α-SMA (stained with red fluorescent color) was determined by immunofluorescent staining of aortic root cross sections and quantified by a computer-assisted image analysis protocol. Scale bar, 100 μm. ^***p < 0.001, ^**p < 0.01, ^*p < 0.05 (n = 3)