Fig. 2

“QN” agarwood mitigated renal inflammation and fibrosis in mice with DKD. A Representative Images of Collagen Staining in Renal Tissue: Masson Staining and Sirius Red Staining, Scale bar = 40X. B The expression levels of Fn1, Col4a1, Kim1, and Tgfb1 mRNA in the renal tissue were assessed. C Protein expression levels of FN, KIM-1, and TGF-β1 in the kidney were examined using WB analysis. D Representative images of renal tissue were obtained through immunohistochemical staining for CD68, Scale bar = 40X. E The expression levels of Ccl2, Il6, and Tnfα mRNA in the renal tissue were assessed. F WB of MCP-1 and IL6 in the kidney. ^###p < 0.001 versus CON group; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 versus DKD group. n.s indicates no statistically significant difference