Fig. 1

OP improves the cell viability and mitochondrial homeostasis in tBHP-induced H9C2 cells. A The chemical structure of OP. B H9C2 cells were treated with OP (5, 10, 20, 40, 80 μM) for 24 h, the cytotoxicity was detected by MTT assay. C H9C2 cells were treated with tBHP (50, 100, 150, 200, 300 μM) for 4 h, the cell viability was detected by MTT assay. D H9C2 cells were pretreated with OP (10, 20, 40 μM) for 2 h and then acted with tBHP (150 μM) for 4 h, the cell viability was detected by MTT assay. E The effect of OP in tBHP- induced changes of Cell morphology. F The LDH release level. G Flow cytometry detected the level of ROS. H Statistics of flow cytometry. I H9C2 cells were stained with DCFH2-DA to detect the ROS level. J H9C2 cells were stained with JC-1 probe to detect the MMP level. All data are expressed as mean ± SEM, n = 3. ^###P < 0.001 vs control group; ^*P < 0.05, ^**P < 0.01, ^***P < 0.005 vs tBHP group