Fig. 2

OP actives Nrf2 signal pathway in tBHP-induced H9C2 cells. A The effect of OP on the protein expressions of Nrf2, Keap1, HO-1, and NQO1 in tBHP-induced H9C2 cells as indicated. B–E The statistics of protein gray value. F Qrt-PCR detected the effect of OP on the mRNA levels of Nrf2, HO-1, and NQO1 in tBHP-induced H9C2 cells. G The Nrf2 protein levels in cytoplasm and nucleus were detected by western blot. H The statistics of Nrf2 protein level. I The level of Nrf2 nuclear translocation was detected by immunofluorescence. J Statistics of the ratio of nuclear and cytoplasmic fluorescence. All data are expressed as mean ± SEM, n = 3. ^#P < 0.05, ^##P < 0.01, ^###P < 0.005 vs Control; ^**P < 0.01, ^***P < 0.005 vs tBHP