Fig. 4

OP binds to TLR4 and regulates MAPK pathway. A Molecular docking data analysis of OP and TLR4 using autodock software. B The CETSA experiment further confirmed the interaction of OP and TLR4 (n = 3). C The statistics of protein levels of (B). D, E Biacore X100 detected the kinetics/Affinity of OP with TLR4. F The effect of OP on the level of TLR4 and the phosphorylation levels of P38, ERK1/2 and JNK in tBHP-induced H9C2 cells (n = 3). G The statistics of protein levels of (F). ^###P < 0.005 vs Control; ^***P < 0.005 vs tBHP (or DMSO)